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关键词： 对口援建新疆班   生物化学   教学  

摘要： 生物化学是一门重要的医学基础课程,对口援建新疆高职学生的心理特点和学习方式使得该课程的教学存在较大难度。该文对对口援建新疆定向培养高职护理班教学过程中存在的问题进行分析,并针对问题提出相应的一些生物化学教学对策。

关键词： 虚拟仿真实验   实验教学改革   混合式教学   生物化学  

摘要： 指出了生物化学虚拟仿真实验突破了传统实验的时空的限制,弥补了传统实体实验耗材高、耗时长、设备紧张、操作繁杂等不足,具有开放、共享、安全的特点。通过按照“虚实结合,能实不虚,优势互补”的原则,构建了虚实结合的混合式实验教学模式,以利于整合优质教学资源,提高学生自主学习能力和实验教学效率,培养创新意识和能力,促进应用型人才的培养质量。

关键词： Color development   Feutrell's early   affect peel composition   juice biochemical properties  

摘要： Color development in 'Feutrell's early' fruit was used to determine the time of harvest, when color changes from dark green to fully orange take place during season of peak maturity. Ten fruits from each replication were harvested on visual observation of their skin color, and were subsequently made ready for biochemical analysis of color determination by using Chromameter. Dark green color to light green fruits were ordered in (1-5) order whereas light orange to deep orange were given (6-10) numbering orders. Triplicate determinations of all samples were used for all the laboratory analysis. Fruit-peel chlorophylls were recorded as more in fruits having color dark green which on the other hand, had gradually decreased in orange colored fruits. The carotenoids, however, have showed the opposite trend. Titratable acidity and ascorbic acid were observed as lessened in green coloration fruits, while the pH, total soluble solids (TSS), total dissolved salts (TDS), and sugar values showed an increasing tendency in orange-colored fruits. Values of reducing sugars and antioxidants were noted as decreased in orange colored fruits while anthocyanins were increased in that produce. Nonetheless, phenolic contents, flavonoids and flavonols were not significantly affected in coloration. Dark green to light green fruits possessed more titratable acidity, ascorbic acid, antioxidant as well as phenolic contents, and were more useful from nutritional point of view. Light orange to deep orange colored fruits had more sweetness, palatability, and tastiness to appeal increased consumer's acceptance.

关键词： 生物化学课程   停课不停学   网络教学   护理专业  

摘要： 为了共同应对新型冠状病毒肺炎的疫情,探索积极有效的线上教学方式,做到“停课不停学”,文章针对现阶段网络教学中存在的问题,提出了提高护理专业生物化学课程网络教学效果的方法,包括细致进行前期备课、互动讨论式直播授课、增强学习社群感等,多措并举提升网络授课的教学效果,保障教学质量。

关键词： Cactus   Opuntia megacantha Salm-Dyck   Cladodes   Acid phosphatase   Purification   Biochemical characterization  

摘要： Acid phosphatase (ACP) plays an important role in regulating phosphate nutrition in plants. Herein, for the first time, a novel ACP fromOpuntiamegacantha Salm-Dyck cladodeswas purified to homogeneity and biochemically characterized. Specific activity of 8.78U/mgwas obtained with 11.29-fold purification and 15% yield. ACPwas purified as monomer with molecular weight of 44 kDa as determined by SDS-PAGE under denaturing and nondenaturing conditions. Optimum pH and temperature for ACP activity was 5.5 and 60 degrees C, respectively. Thermodynamic parameters (E-a, Delta H, Delta G and Delta S) were also determined. ACP activitywas stimulated by Ca2+, strongly inhibited by Cu2+ and Fe3+, and moderately inhibited by Mg2+ and Zn2+. Br-, CN-, F-, I- and N-3(-) weakly inhibited ACP activity, where more than 70% of enzyme activity was remained at 5 mM. In addition, effect of beta-ME, Cys, DTT, EDTA, H2O2, PMSF, SDS and TX-100 on ACP activity was investigated. k(m), V-max, k(cat) and k(cat)/k(m) of ACP for p-NPP were found to be 0.09 mM, 2.75 U/mL, 9.60 s(-1) and 106.67 s(-1) mM(-1), respectively. The biochemical properties of ACP from Opuntia megacantha Salm-Dyck cladodes provide novel features with other plant ACPs and basic knowledge of ACP in Opuntia species. (C) 2020 Elsevier B.V. All rights reserved.

关键词： 高职   食品生物化学   激发   课堂技巧  

摘要： “食品生物化学”是高职食品类专业一门重要的核心基础课程,但因其为多种学科的交叉学科,内容抽象、分子式多、代谢过程复杂等特点给学生的学习造成一定的困扰。结合高职院校学情,充分激发学生的积极性和主动学习能力,从而提升高职院校学生“食品生物化学”课程的教学效果,这也是促进学生学好“食品生物化学”的重要途径。

关键词： 生物化学   实验教学   微课   微信  

摘要： 新媒体资源对教育行业的影响日趋增加,但是高校教育更新相对滞后,我们希望探索生物化学实验微课的新形式,辅助传统实验教学,借助新媒体资源提升学生的学习兴趣,建设学生和教师沟通的新模式。因此,就微课教学模式在生物化学中的应用进行探讨与思考具有重要意义。

关键词： alpha-amylase   Arabian balsam   encapsulation   Antioxidant capacity   Purification  

摘要： In this study, Arabian balsam alpha-amylase was purified using the three-step purification method with 9.8-fold pu-rification and 7% recovery. The purified alpha-amylase's molecular weight was 85 kDa. Calcium alginate incorporated with iron (III) oxide nanoparticles was applied as an immobilizing support for alpha-amylase. The immobilized alpha-amylase was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. In addition, the optimum conditions for immobilization efficiency, immobilization time, reusability, kinetic parameters, and the effect of pH for the immobilization process were examined. After storage, about 87% of the initial activity was maintained at 4 degrees C for 60 days. The immobilized enzyme exhibited enhanced stability compared to the soluble enzyme in relation to pH and temperature. The immobilized enzyme provided the following kinetic variables: 0.455 mg/mL, 4050 s(-1), 28.57 mu mol maltose/mL, and 8900 s(-1) mg(-1) mL for Km, kcat, Vmax, and kcat/Km, respectively, compared with 1.798 mg/mL, 5980 s(-1), 42.19 mu mol maltose/mL, and 3326 s(-1) mg(-1) mL for the soluble enzyme. The total phenolic contents of the soluble and immobilized alpha-amylase-treated wheat kernels were increased by 1.26 and 1.31 fold, respectively. Purified alpha-amylase from Arabian balsam can thus be successfully used to enhance the antioxidant capacity of cereals. (C) 2020 Elsevier B.V. All rights reserved.

关键词： 微助教   生物化学   教学改革  

摘要： 微助教辅助生物化学课堂教学具有良好的效果,其明显优势在于快捷的考勤方式,实时的互动平台,方便的过程评价,随时随地的课程复习。微助教的应用可提升同学们的参与感和成就感,也可以促使教师根据反馈情况,及时调整教学进度,改进教学方法,真正做到教学相长。

关键词： 课程思政   思政元素   融入策略   《医学生物化学》课程  

摘要： 课程思政是全面落实立德树人根本任务的重要举措。近日,教育部提出了全面推进高校课程思政建设的要求和指导方案,专业课教师应该充分认识课程思政的重要意义,主动地去探索如何将思政元素有机地融入到专业知识的教学中,更好地实现育人目标。本文以《医学生物化学》课程为例,探讨了不同的视角下课程思政元素的融入策略。