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关键词： Plant growth promoting bacteria   Cadmium tolerance   Maize cultivars   Morpho-physio-biochemical attributes   Phytostabilization  

摘要： Cadmium is a phytotoxic metal which threatens the global food safety owing to its higher retention rates and non-biodegradable nature. Optimal study of microbe-assisted bioremediation is a potential way to minimize the adversities of Cd on plants. Current study was aimed to isolate, identify and characterize Cd tolerant PGPBs from industrially contaminated soil and to evaluate the potential of plant-microbe synergy for the growth augmentation and Cd remediation. The Acinetobacter sp. SG-5, identified through 16S rRNA gene sequence analysis, was able to tolerate 1000 mg/l of applied Cd stress and ability of in vitro indole-3-acetic acid production, phosphate solubilization, as well as 1-aminocyclopropane-1 -carboxylic acid deaminase activity. A Petri plate experiment was designed to investigate the impact of Acinetobacter sp. SG-5 on applied Cd toxicity (0, 6, 12, 18, 24, 30 mu M) in maize cultivars (3062-Cd tolerant, 31P41-Cd susceptible). Results revealed that non-inoculated maize plants were drastically affected with applied Cd treatments for growth, antioxidants and mineral ions acquisition predominantly in susceptible cultivar (31P41). PGPB inoculation positively influenced the maize growth by enhanced anti-oxidative potential coupled with optimum level of nutrients (K, Ca, Mg, Zn). Analysis of morphophysio-biochemical traits after PGPB application revealed that substantial Cd tolerance was acquired by susceptible cv. 31P41 than tolerant cv. 3062 under applied Cd regimes. Research outcomes may be important for understanding the growth responses of Cd susceptible and tolerant maize cultivars under Acinetobacter sp. SG-5 inoculation and likely to provide efficient approaches to reduce Cd retention in edible plant parts and/or Cd bioremediation.

摘要： High-throughput screening (HTS) provides starting chemical matter in the adventure of developing a new drug. In this review, we survey several HTS methods used today for hit identification, organized in two main flavors: biochemical and cell-based assays. Biochemical assays discussed include fluorescence polarization and anisotropy, FRET, TR-FRET, and fluorescence lifetime analysis. Binding-based methods are also surveyed, including NMR, SPR, mass spectrometry, and DSF. On the other hand, cell-based assays discussed include viability, reporter gene, second messenger, and high-throughput microscopy assays. We devote some emphasis to high-content screening, which is becoming very popular. An advisable stage after hit discovery using phenotypic screens is target deconvolution, and we provide an overview of current chemical proteomics, in silico, and chemical genetics tools. Emphasis is made on recent CRISPR/dCas-based screens. Lastly, we illustrate some of the considerations that inform the choice of HTS methods and point to some areas with potential interest for future research.

关键词： Characterization   Grape   Historical cultivars   Phytochemicals  

摘要： Northeastern part of Turkey is rich in terms of autochthonous grape cultivars. In the region Yusufeli district has special importance for historical cultivation of grapevine. This small district has over fourteen local grape cultivars. Present study describe morphological and biochemical characteristics of nine autochthonous grape cultivars grown in Yusufeli district in Turkey. The standard Turkish grape cultivar 'Cavus' was also included experiment to make comparison with local ones. Bunch size, berry color, berry shape and usage are the searched main morphological characteristics. Phenolic compounds, organic acids, vitamin C and specific sugars were the main biochemical parameters. We found a wide variation among both morphological and biochemical characteristics. The cultivars showed low to medium bunch size. Chlorgenic acid (2.231-4.811 & x202f;mg/L), syringic acid (1.027-5.426 & x202f;mg/L) and rutin (1.013-1.129 & x202f;mg/L) were found the highest content in berries of cultivars. As expected, tartaric acid that characteristic for grape berries was the dominant (ranged from 1.826 to 3.336 & x202f;g/L) and followed by malic acid (ranged from 1.089 to 2.344 & x202f;g/L). The all autochthonous grape cultivars had glucose the highest concentration as major sugar and followed by fructose. It can be concluded that morphological and biochemical characteristics analyzed could be useful to give information about selection of the promising grape cultivars for breeders.

关键词： Chickpea   Wild relatives   Helicoverpa armigera   Antibiosis   Biochemical mechanism of resistance  

摘要： Efforts are being made to develop chickpea varieties with resistance to the pod borer, Helicoverpa armigera for reducing pesticide use and minimizing the extent of losses due to this pest. However, only low to moderate levels of resistance have been observed in the cultivated chickpea to this polyphagous pest. Hence, it is important to explore wild relatives as resistance sources to develop insect-resistant cultivars. Therefore, we studied different biochemical components that confer resistance to H. armigera in a diverse array of wild relatives of chickpea. Accessions belonging to wild relatives of chickpea exhibited high levels of resistance to H. armigera as compared to cultivated chickpea genotypes in terms of lower larval survival, pupation and adult emergence, decreased larval and pupal weights, prolonged larval and pupal developmental periods and reduced fecundity of the H. armigera when reared on artificial diet impregnated with lyophilized leaf powders. Amounts of proteins and phenols in different accessions of chickpea wild relatives were significantly and negatively correlated with larval weight, pupation and adult emergence. Phenols showed a negative correlation with pupal weight and fecundity, but positive correlation with pupal period. Total soluble sugars showed a negative correlation with larval period, but positive correlation with pupation and pupal weight, while tannins showed a positive correlation with larval weight, pupation and adult emergence. The flavonoid compounds such as chlorogenic acid, ferulic acid, naringin, 3,4-dihydroxy flavones, quercetin, naringenin, genistein, biochanin-A and formononetin that were identified through HPLC fingerprints, exhibited negative effects on survival and development of H. armigera reared on artificial diet impregnated with lyophilized leaf powders. The wild relatives with diverse mechanisms of resistance conferred by different biochemical components can be used as sources of resistance in chickpea breeding

关键词： High resolution hyperspectral   J(max)   N:P ratio   Nutrient limitation   Physically based models   Radiative transfer   Reflectance   V-cmax  

摘要： The prediction of carbon uptake by forests across fertility gradients requires accurate characterisation of how biochemical limitations to photosynthesis respond to variation in key elements such as nitrogen (N) and phosphorus (P). Over the last decade, proxies for chlorophyll and photosynthetic activity have been extracted from hyperspectral imagery and used to predict important photosynthetic variables such as the maximal rate of carboxylation (V-cmax) and electron transport (J(max)). However, little research has investigated the generality of these relationships within the nitrogen (N) and phosphorus (P) limiting phases, which are characterised by mass based foliage ratios of N:P <= 10 for N limitations and N:P > 10 for P limitations. Using measurements obtained from one year old Pinus radiata D. Don grown under a factorial range of N and P treatments this research examined relationships between photosynthetic capacity (V-cmax, J(max)) and measured N, P and chlorophyll (Chl(a+b)). Using functional traits quantified from hyperspectral imagery we then examined the strength and generality of relationships between photosynthetic variables and Photochemical Reflectance Index (PRI), Sun-Induced Chlorophyll Fluorescence (SIF) and chlorophyll a + b derived by radiative transfer model inversion. There were significant (P < .001) and strong relationships between photosynthetic variables and both N (R-2 = 0.82 for V-cmax;R-2 = 0.87 for J(max)) and Chl(a+b) (R-2 = 0.85 for V-cmax;R-2 = 0.86 for J(max)) within the N limiting phase that were weak (R-2 < 0.02) and insignificant within the P limiting phase. Similarly, there were significant (P < .05) positive relationships between P and photosynthetic variables (R-2 = 0.50 for V-cmax;R-2 = 0.58 for J(max)) within the P limiting phase that were insignificant and weak (R-2 < 0.33) within the N limiting phase. Predictions of photosynthetic variables using Chl(a+b) estimated by model inversion were significant (P < .001), positive a

摘要： A 019

关键词： APX activity   Chlorophyll contents   Flooding stress   Gene expression   Sweetpotato  

摘要： Sweetpotato [Ipomoea batatas (L.) Lam] serves as a sustainable food source and ensures nutrition security in the face of climate change. Recently, farmers have developed increased interest in replacing rice with sweetpotato in paddy fields for higher income. However, sweetpotato is more susceptible to flooding stress than other abiotic stresses including drought and salinity. Here, we selected flooding tolerant sweetpotato cultivars based on biochemical characterization. Young seedlings of 33 sweetpotato cultivars were subjected to flooding stress for 20 days, and Yeonjami (YJM) was identified as the most flooding tolerant sweetpotato cultivar. Plant growth and biochemical characteristics of YJM were compared with those of Jeonmi (JM), a flooding sensitive sweetpotato cultivar. Under flooding stress, YJM showed higher content of chlorophyll and lower inhibition of plant height and fibrous root length than JM. Biochemical characterization revealed that although malondialdehyde and hydrogen peroxide contents were increased in fibrous roots of both cultivars, the amount of increase was 4-fold lower in YJM than in JM. Additionally, leaves of YJM showed higher ascorbate pemxidase activity than those of JM under flooding stress. Our results suggest that high membrane stability and antioxidant capacity are important flooding tolerance factors in sweetpotato. Furthermore, several flooding tolerance-related genes involved in starch and sucrose metabolism, fermentation, and cell wall loosening showed earlier induction and higher transcript levels in YJM leaves and fibrous roots than in JM tissues under flooding stress. Thus, phenotypic and biochemical characterization suggests that YJM could be used as a flooding tolerant sweetpotato cultivar.

关键词： atorvastatin   kidney stone   statin   testosterone   urolithiasis  

摘要： Objective To investigate whether the use of statins would alter 24-h urine biochemistry in male patients with calcium kidney stones. Methods We prospectively recruited 78 male patients with calcium kidney stones between May 2017 and December 2017, and 30 male controls with matching sex and age, but without kidney stones. All patients were classified into higher- and lower-risk groups of atherosclerotic cardiovascular disease according to the American College of Cardiology/American Heart Association guidelines. Atorvastatin 20 mg per day was prescribed for 12 weeks to the higher risk patients. For kidney stone group, 24-h urine collections were carried out before and after statin therapy. Results A total of 78 patients and 30 controls were included. Higher-risk patients had significantly higher urine uric acid and calcium levels than lower-risk patients. After atorvastatin treatment for 12 weeks, urine citrate significantly increased (P < 0.001) accompanied with increased urine pH (P < 0.001), whereas urine uric acid significantly decreased after treatment. Although urine oxalate significantly increased after treatment (P = 0.037), we did not find any significant difference in urine calcium, ion activity product of calcium oxalate and ion activity product of calcium phosphate. Conclusion These findings suggest that atorvastatin administration might increase urinary citrate and decrease urinary uric acid in patients with calcium kidney stones and dyslipidemia.

关键词： Alpha-glucosidase   Antimicrobial activity   Capsaicin   Carotenoid   Pepper seed oil   Phenolics  

摘要： In this study, the physicochemical, functional, and antimicrobial properties of pepper seed oil (PSO) were determined. PSO was subjected to differential scanning calorimeter (DSC), fatty acid composition, carotenoid, capsaicin, and tocopherol analyses. LC-ESI-MS/MS and NMR were used to characterize and quantify phytochemicals. Resveratrol, luteolin, and 4-hydroxycinnamic acid were the principal phenolics in PSO. A high concentration of unsaturated fatty acids (85.3%), especially linoleic acid (73.7%) is present in PSO. Capsaicin, dihydrocapsaicin, alpha-tocopherol, delta-tocopherol, zeaxanthin, and capsanthin were determined in PSO at concentrations of 762.92, 725.73, 62.40, 643.23, 29.51, 16.83 ppm, respectively. PSO displayed inhibitory activity against alpha-glucosidase rather than alpha-amylase. The antimicrobial activity of PSO was tested against Escherichia coli, Staphylococcus aureus subsp. aureus, Aspergillus brasiliensis and Candida albicans. The antimicrobial potential of PSO was expressed as minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and inhibition zone (IZ) diameter. Polyunsaturated fatty acid, capsaicin, carotenoid, tocopherol, resveratrol contents;the antioxidant, alpha-glucosidase inhibitory and antimicrobial activities of PSO indicated its nutritional value and health promoting nature for the well-being of humans.

关键词： Acid phosphatase   Catalase   Glycine max (L.) Merrill   Glycine sofa Sieb. & Zucc.   Multiple forms   Oxidative stress   Peroxidase   Ribonuclease specific activity  

摘要： Agricultural production is becoming increasingly dependent on the environmental factors that alter soil properties, plant productivity, and product quality. Environment pollution caused by heavy metals because of human activities are among the most dangerous pollutants on the biosphere. Here, we have studied the biochemical adaptation of wild and cultivated soybeans to the simulated effects of lead nitrate and lead acetate. Lead in the form of acetate had a relevant toxic effect, as evidenced by a significant increase in the concentration of malonic dialdehyde in the treated samples relative to control samples. Catalase and peroxidase, possibly performing a signaling function, are involved in the adaptation to the toxicity of Pb salts. The studied Pb salts showed a predominant stimulating effect on the specific activity of acid phosphatases in cultivated soybean, while the ribonuclease activity changed in both Glycine species. Moreover, in wild soybean, it was mostly suppressive, except for the first day. We found that the electrophoretic spectra of acid phosphatases of soybean seedlings was highly stabile, while that of ribonucleases varied depending on the salt. On the seventh day of exposure, lead nitrate caused a decrease in the specific activity of the studied hydrolases of seedlings of cultivated and wild soybeans. A change in the number or electrophoretic mobility of multiple forms of enzymes during treatment with Pb salts was revealed, which indicates the adaptation of the plants at the molecular genetic level. These results imply that the observed enzymes can be used as sensitive indicators for predicting the effects of heavy metals on soybean.