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关键词： N-linked glycosylation   endoplasmic reticulum   hypoglycosylation   yeast OST subunits  

摘要： Asparagine-linked glycosylation is a highly conserved protein modification reaction that occurs in all eukaryotes. The initial stage in the biosynthesis of N-linked glycoproteins, catalyzed by the enzyme oligosaccharyltransferase (OST), involves the transfer of a preassembled high-mannose oligosaccharide from a dolichol-linked oligosaccharide donor onto asparagine acceptor sites in nascent proteins in the lumen of the rough endoplasmic reticulum, Biochemical, molecular biological, and genetic studies conducted during the past 5 years have resulted in an explosive growth in our knowledge concerning the OST, Although the basic biochemical properties of the enzyme were determined more than a decade ago using intact microsomal membranes, recent studies provide novel insight into the catalytic mechanism of the enzyme, The OST was recently purified as a large heteroligomeric membrane protein complex;the sequences of many of the subunits have been determined from both fungal and vertebrate sources, Consistent with the evolutionary conservation of N-linked glycosylation, protein sequence comparisons reveal significant homologies between vertebrate, invertebrate, plant, and fungal OST subunits, Yeast molecular genetic methods have been instrumental in the functional characterization of the OST subunits, and have proven to be powerful tools for the identification of novel gene products that influence oligosaccharide transfer in vivo.

关键词： tRNA   转移RNA   生物化学   分子生物学  

摘要： 是生物化学，还是分子生物学？──发现ｔＲＮＡ的故事Ｍ．Ｂ．Ｈｏａｇｌａｎｄ关键词ｔＲＮＡ，生物化学，分子生物学在本世纪４０年代后期和５０年代早期，生物化学家和分子生物学家中的开拓者们正在不同的方向上进行科研工作。在我成长起来的那个生物化学家圈子里，人...

关键词： 生物学教学   第二课堂活动   新教学体系   毕业论文   研究生   五层次   教学大纲   学术水平   生物化学与分子生物学   实验室建设  

摘要： 勇于探索构建新的生物学教学体系黄永芬，郭桂云，汪清胤，冯树丹，弭小菊传统的高师生物化学教学，按原教学大纲规定为专业基础课，在大学二年级开课。选修课，仿综合性大学设课体系，结合我校的具体条件开设一门课，“核酸生化”“蛋白质生物化学”。研究这个教学体系是...

关键词： 脂蛋白   载脂蛋白   心脏血管疾病  

摘要： 脂蛋白（ａ）［Ｌｐ（ａ）］是一种与低密度脂蛋白类似的脂蛋白，其特殊处在于多含一种具明显多态性的糖蛋白──载脂蛋白（ａ）［Ａｐｏ（ａ）］。Ａｐｏ（ａ）与血浆纤溶酶原有很大同源性。Ｌｐ（ａ）由肝合成，其分解可能主要经非特异途径。Ａｐｏ（ａ）大小及血浆Ｌｐ（ａ）浓度主要由Ａｐｏ（ａ）基因决定。Ｌｐ（ａ）易沉积于血管壁，并可促进平滑肌细胞生长及抑制纤维蛋白溶解，这可能是其促动脉粥样硬化和血栓形成的机理所在。Ｌｐ（ａ）的生理功能尚不清楚。

关键词： Amino Acids   Amino Acyl-tRNA Synthetases   Autobiography   History   20th Century   Protein Biosynthesis   RNA   RNA   Transfer   Transfer RNA Aminoacylation  

摘要： Hoagland comments on the merging of biochemistry and molecular biology and his place in the merger. The most memorable experience of his career was discovering soluble RNA--the physical link between activated amino acids and their ordered arrangement in protein molecules.

关键词： 中科院   分子生物学   实验室   国家重点实验室  

关键词： 化学信号   植物   微生物   基因表达   转导  

摘要： 本文综述了近年来植物－微生物化学信号物质的发现和鉴定、化学信号传导的机理、相关基因的表达和调控等方面的研究进展；并提出存在的问题。

ISBN: (数字)9780080581262 ISBN: (纸本)9780120329359

摘要： Each volume of Advances in Pharmacology provides a rich collection of reviews on timely topics. Emphasis is placed on the molecular bases of drug action, both applied and experimental. This volume contains chapters that address diverse but interrelated areas pertaining to the chemistry, biochemistry, molecular biology, and pharmacology of nitric oxide in mammalian cells. The contents form a comprehensive treatise of factors influencing the control of nitric oxide production in various cell *** comprehensive coverage of the chemical properties of nitric oxide and how they form the basis for the multifaceted biological actions for nitric oxideContains the most current and detailed documentation of the properties and regulation of nitric oxide synthasesProvides the most up-to-date review of inhalational nitric oxide therapy for treatment of respiratory dysfunction

关键词： Medical   Parasitology  

ISBN: (数字)9780080527888 ISBN: (纸本)9780124733459

摘要： The study of parasitic organisms at the molecular level has yielded fascinating new insights of great medical, social, and economical importance, and has pointed the way for the treatment and prevention of the diseases they cause. Biochemistry and Molecular Biology of Parasites presents an up-to-date account of this modern scientific discipline in a manner that allows and encourages the reader to place the biochemistry and molecular biology of these organisms in their biological context. The chapters are cross-referenced and grouped in an arrangement that provides a fully integrated whole, and permits the reader to create a composite of the biochemical function of these organisms. Individual chapter includes those devoted to metabolism, in both aerobic and anaerobic protozoa; antioxidant mechanisms; parasite surfaces; organelles; invasion mechanisms; and chemotherapy. The helminths are discussed not only from the point of view of their cellular biochemistry and metabolism, but also with respect to both their integrated functions such as neurochemistry, structure and functions of surfaces, and reproduction. Written by expert investigators, this book will be of interest to all experienced researchers, graduate students, and to the newcomer eager to become familiar with the biochemistry and molecular biology of parasites.

关键词： 生物化学   分子生物学   研究报告   发展   中国  

ISBN: (纸本)7030048040

摘要： 本书可供生物化学与分子生物学以及有关学科的科技领导干部，科技管理人员和科技工作者参考。